Glucose Biosensor Based on Dendritic Gold Nanostructures Electrodeposited on Graphite Electrode by Different Electrochemical Methods

In this research, we have demonstrated a one-step electrochemical deposition of dendritic gold nanostructures (DGNs) on graphite rod (GR) electrode without any template, seeds, surfactants, or stabilizers. Three methods, namely, constant potential amperometry (CPA), pulse amperometry, and differential voltammetry, were used for DGN synthesis GR further application in enzymatic glucose biosensors. Formed nanostructures, including DGNs, characterized by field emission scanning electron microscopy. The optimal concentration HAuCl4 (6.0 mmol L?1), duration DGNs (400 s), electrodeposition (?0.4 V), the best method (CPA) determined experimentally. Then enzyme, oxidase, was adsorbed surface covalently cross-linked with glutaraldehyde vapor. biosensor based electrodeposited at conditions modified oxidase showed quick response (less than 3 high saturation current (291 ?A), appropriate linear range (up to 9.97 L?1 glucose, R2 = 0.9994), good repeatability (RSD 2.4, 2.2 1.5% 2, 30, 97 glucose), low limit detection (0.059 L?1, S/N 3) stability. Additionally, could be successfully applied determination real samples accuracy. These results proved principle development as basis investigations.